KAPA SYBR® FAST One-Step qRT-PCR Kits
qRT-PCR سریع یک مرحله ای به کمک سایبر
کیت یک مرحله ای qRT-PCR بجز رنگ سایبر حاوی M-MuLV Reverse Transcriptase باز دارنده RNase و پلی مراز تراریخته نیز می باشد.این کیت به گونه طراحی شده که با کارایی بالا و بازده بسیار عالی در شرایط سخت در یک مرحله بیان مناسبی را از ژنهای مختلف نشان دهد.
کاربردها:
• تجزیه و تحلیل بیان ژن
• Microarray
• آنالیز ژنهای سرکوب شده
• تحقیقات RNAi and miRNA
شرایط نگهداری: در دمای -20درجه سانتی گراد کیت یک مرحله ای qRT-PCR میتواند تا 18ماه حفظ شود
For 100 x 20 uL reactions. Kits include 50X KAPA RT Mix (40 µL) and 10 mM dUTP (40 µL). Supplied with separate 50X ROX High and 50X ROX Low (200 µL each). | KAPA SYBR® FAST One-Step qRT-PCR Universal (1 mL) | KK4650 |
For 500 x 20 uL reactions. Kits include 50X KAPA RT Mix (200 uL). 10 mM dUTP (200 µL). Supplied with separate 50X ROX High and 50X ROX Low (200 µL each). | KAPA SYBR® FAST One-Step qRT-PCR Universal (5 mL) | KK4651 |
For 1000 x 20 µL reactions. Kits include 50X KAPA RT Mix (2 x 200 µL) and 10 mM dUTP (2 x 200 µL). Supplied with separate 50X ROX High and 50X ROX Low (2 x 200 µL each). | KAPA SYBR® FAST One-Step qRT-PCR Universal (2 x 5 mL) | KK4652 |
For 100 x 20 uL reactions. Kits include 50X KAPA RT Mix (40 µL) and 10 mM dUTP (40 µL). ROX is included in the master mix at an optimal concentration for most ABI qPCR cyclers | KAPA SYBR® FAST One-Step qRT-PCR ABI Prism (1 mL) | KK4660 |
For 500 x 20 uL reactions. Kits include 50X KAPA RT Mix (200 uL). 10 mM dUTP (200 µL). ROX is included in the master mix at an optimal concentration for most ABI qPCR cyclers | KAPA SYBR® FAST One-Step qRT-PCR ABI Prism (5 mL) | KK4661 |
For 1000 x 20 µL reactions. Kits include 50X KAPA RT Mix (2 x 200 µL) and 10 mM dUTP (2 x 200 µL). ROX is included in the master mix at an optimal concentration for most ABI qPCR cyclers | KAPA SYBR® FAST One-Step qRT-PCR ABI Prism (2 x 5 mL) | KK4662 |
For 100 x 20 uL reactions. Kits include 50X KAPA RT Mix (40 µL) and 10 mM dUTP (40 µL). Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. | KAPA SYBR® FAST One-Step qRT-PCR Bio-Rad iCycler (1 mL) | KK4670 |
For 500 x 20 uL reactions. Kits include 50X KAPA RT Mix (200 uL). 10 mM dUTP (200 µL). Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. | KAPA SYBR® FAST One-Step qRT-PCR Bio-Rad iCycler (5 mL) | KK4671 |
For 1000 x 20 µL reactions. Kits include 50X KAPA RT Mix (2 x 200 µL) and 10 mM dUTP (2 x 200 µL). Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. | KAPA SYBR® FAST One-Step qRT-PCR Bio-Rad iCycler (2 x 5 mL) | KK4672 |
For 100 x 20 uL reactions. Kits include 50X KAPA RT Mix (40 µL) and 10 mM dUTP (40 µL). Optimized for use with the LightCycler 480. | KAPA SYBR® FAST One-Step qRT-PCR Roche LightCycler® 480 (1 mL) | KK4680 |
For 500 x 20 uL reactions. Kits include 50X KAPA RT Mix (200 uL). 10 mM dUTP (200 µL). Optimized for use with the LightCycler 480. | KAPA SYBR® FAST One-Step qRT-PCR Roche LightCycler® 480 (5 mL) | KK4681 |
For 1000 x 20 µL reactions. Kits include 50X KAPA RT Mix (2 x 200 µL) and 10 mM dUTP (2 x 200 µL). Optimized for use with the LightCycler 480. | KAPA SYBR® FAST One-Step qRT-PCR Roche LightCycler® 480 (2 x 5 mL) | KK4682 |