E.coli strain that carries the cloned EcoR V gene from Escherichia coli
GM-buffer W + BSA, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
:Inactivation 20 minutes
:Enzyme activity in % of maximum