E.coli strain that carries the cloned Hind II gene from Haemophilus influenzae
GM-buffer G + BSA, (10mM Tris-HCl (pH 7.6 at 25℃); 10 mM MgCl2; 50 mM NaCl; 1mM DTT + 100 μg/ml BSA.)
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
After 10-fold overdigestion with enzyme 60% of DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
:Inactivation 20 minutes
:Enzyme activity in % of maximum