E.coli strain that carries the cloned Hpa I gene from Haemophilus parainfluenzae
GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1mM DTT.)
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
After 5-fold overdigestion with enzyme about 60% of DNA fragments can be ligated and recut.
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 37°C.
:Inactivation 20 minutes
:Enzyme activity in % of maximum