:Origin Bacillus stearothermophilus AF :Concentration 5000 – 20000, u.a./ml :Recognition site C/TTAAG GAATT/C :Reaction buffer GM-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. :Storage […]
:Origin E.coli strain that carries the cloned Afe I gene from Alcaligenes faecalis T2774 :Concentration 10000, u.a./ml :Recognition site AGC/GCT TCG/CGA :Reaction buffer GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM […]
:Origin E. coli strain that carries the cloned Acu I gene from Acinetobacter calcoaceticus SRW4 :Concentration 500 – 2000, u.a./ml :Recognition site CTGAAG(N)16/ GACTTC(N)14/ :Reaction buffer GM-buffer Y + SAM + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 0.01 mM S-adenosylmethionine + […]