:Origin Bacillus stearothermophilus AU :Concentration 20000, u.a./ml :Recognition site T/GTACA ACATG/T :Reaction buffer GM-buffer W, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol. :Storage temperature -20°C :Ligation […]
:Origin Bacillus stearothermophilus 118 :Concentration 2000-5000, u.a./ml :Recognition site R/CCGGY YGGCC/R :Reaction buffer GM-buffer O, (50mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) :Storage conditions 10 mM KH2PO4 (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol. Storage temperature: -20°C :Ligation […]
:Origin :Bacillus stearothermophilus 3D :Concentration 10000, u.a./ml :Recognition site GCAATGNN/ CGTTAC/NN :Reaction buffer GM-buffer G, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol :Storage temperature -20°C :Ligation After 10-fold overdigestion […]