BsrB I (AccBS I)

:Origin Acinetobacter calcoaceticus BS :Concentration 5000 – 20000, u.a./ml :Recognition site GAG/CGG CTC/GCC :Reaction buffer GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. […]

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BspM I (BfuA I, Acc36 I)

:Origin Acinetobacter calcoaceticus 36 :Concentration 2000-5000, u.a./ml :Recognition site ACCTGC(N)4/ TGGACG(N)8/ :Reaction buffer GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol. :Storage temperature […]

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BspE I (Bsp13 I)

:Origin Bacillus species 13 :Concentration 10000 – 20000, u.a./ml :Recognition site T/CCGGA AGGCC/T :Reaction buffer GM-buffer 2K, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol :Storage […]

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