:Origin Micrococcus halobius SD :Concentration 5000 – 10000, u.a./ml :Recognition site GDGCH/C C/HCGDG :Reaction buffer GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. :Storage temperature -20°C :Ligation […]
:Origin Bacillus stearothermophilus FI :Concentration 1000, u.a./ml :Recognition site GGGAC(N)10/ CCCTG(N)14/ :Reaction buffer GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA. ) :Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM […]
:Origin Bacillus species AC :Concentration 2000 – 5000, u.a./ml :Recognition site C/CGC GGC/G :Reaction buffer ; GM-buffer O + BSA, (50 mM Tris-acetate (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ ml BSA.) :Storage conditions 10 mM KH2PO4 (pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 […]