:Origin Isolatedfrom an E.coli strain that carries the cloned Bgl I gene from Bacillus globigii :Concentration 10000, u.a./ml :Recognition site A/GATCT TCTAG/A :Reaction buffer GM-buffer O , (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2 ; 100 mM NaCl; 1 mM DTT .) :Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; […]
:Origin Bacillus globigii :Concentration 5000 – 10000, u.a./ml :Recognition site GCCNNNN/NGGC CGGN/NNNNCCG :Reaction buffer GM-buffer 2W , (20 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2 ; 200 mM NaCl; 1 mM DTT .) :Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% […]
:Origin Bacillus stearothermophilus V1 :Concentration 1000 – 2000, u.a./ml :Recognition site GCAGC(N)8/ CGTCG(N)12/ :Reaction buffer GM-buffer G , (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2 ; 50mM NaCl; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol :Storage temperature -20°C […]