Origin: E.coli strain that carries the cloned Mnl I gene from Moraxella nonliquefaciens Concentration: 10000, u.a./ml Recognition site: CCTC(N)7/ GGAG(N)6/ Reaction buffer: GM-buffer G + BSA, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT + 100 μg/ml BSA) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; […]
Origin: Micrococcus species 20 Concentration: 1000 – 3000, u.a./ml Recognition site: TGG/CCA ACC/GGT Reaction buffer: GM-buffer W + BSA, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA) Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml […]
Origin: E.coli strain that carries the cloned Mse I gene from Thermus ruber 9 Concentration: 20000, u.a./ml Recognition site: T/TAA AAT/T Reaction buffer: GM-buffer W, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 […]