Origin: Planococcus citreus S Concentration: 500 – 2000, u.a./ml Recognition site: GCTCTTC(N)1/ CGAGAAG(N)4/ Reaction buffer: GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Storage temperature: -20°C Ligation: […]
Origin: E.coli strain that carries the cloned Sal I gene from Streptomyces albus Concentration: 10000, u.a./ml Recognition site: G/TCGAC CAGCT/G Reaction buffer: GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl;1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; […]
Origin: Streptomyces fradiae 303 Concentration: 5000 – 20000, u.a./ml Recognition site: CCGC/GG GG/CGCC Reaction buffer: GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage temperature: -20°C Ligation: […]