Origin: Micrococcus roseus N0 Concentration: 2000 – 10000, u.a./ml Recognition site: G/CCGGC CGGCC/G Reaction buffer: GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage temperature: -20°C Ligation: […]
Origin: E.coli strain that carries the cloned Nde I gene from Flavobacterium aquatili ND Concentration: 10000 – 20000, u.a./ml Recognition site: CA/TATG GTAT/AC Reaction buffer: GM-buffer Y + BSA, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH […]
Origin: Bacillus species 19 Concentration: 10000 – 20000, u.a./ml Recognition site: C/CATGG GGTAC/C Reaction buffer: GM-buffer 2W + BSA, (20 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; […]