Origin: Acinetobacter calcoaceticus B7 Concentration: 5000, u.a./ml Recognition site: CCANNNN/NTGG GGTN/NNNNACC Reaction buffer: GM-buffer G, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage temperature: -20°C […]
Origin: Zoogloea ramigera SCA Concentration: 10000-20000, u.a./ml Recognition site: AGT/ACT TCA/TGA Reaction buffer: GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acatate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA .) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM […]
Origin: Bacillus stearothermophilus NS Concentration: 10000, u.a./ml Recognition site: RCATG/Y Y/GTACR Reaction buffer: GM-buffer B + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol. […]