Origin: Isolated from Streptomyces species Bf61 Concentration: 5000, u.a./ml Recognition site: CCTGCA/GG GG/ACGTCC Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acatate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% […]
Origin: Arthrobacter species S9 Concentration: 10000 – 30000, u.a./ml Recognition site: G/GNCC CCNG/G Reaction buffer: GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage […]
Origin: Vibrio nereis 18 Concentration: 10000, u.a./ml Recognition site: G/TGCAC CACGT/G Reaction buffer: GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol Storage temperature: -20°C Ligation: […]