Origin: E. coli strain that carries Vsp I gene from Vibrio species 343 Concentration: 10000, u.a./ml Recognition site: AT/TAAT TAAT/TA Reaction buffer: GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM KPO4 (pH 7.2); 50 mM NaCl; 0.1 mM EDTA; 7 mM […]
Origin: Arthrobacter citreus Concentration: 10000-20000, u.a./ml Recognition site: R/AATTY YTTAA/R Reaction buffer: GM-buffer W + BSA,(10 mM Tris-HC l(pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 50% glycerol Storage temperature: […]
Origin: Acetobacter pasteurianus Concentration: 10000, u.a./ml Recognition site: GGGCC/C C/CCGGG Reaction buffer: GM-buffer Y + BSA, (33mM Tris-acetate(pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM poatassium acetate; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; […]