Origin: Microbacterium arborescens SE Recognition site: A/CCWGGT TGGWCC/A Concentration: 1000 – 5000, u.a./ml Reaction buffer: GM-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 […]
Origin: Bacillus stearothermophilus SF Concentration: 2000 – 5000, u.a./ml Recognition site: C/TRYAG GAYRT/C Reaction buffer: GM-buffer O + BSA, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100μg/ml BSA .) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; […]
Origin: E.coli strain that carries the cloned Sfi I gene from Streptomyces fimbriatus Concentration: 10000, u.a./ml Recognition site: GGCCNNNN/NGGCC CCGGN/NNNNCCGG Reaction buffer: GM-buffer G + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT + 100μg/ml BSA .) Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM […]