Origin: E.coli strain that carries the cloned Pvu II gene from Proteus vulgaris Concentration: 10000, u.a./ml Recognition site: CAG/CTG GTC/GAC Reaction buffer: GM-buffer G + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT + 100μg/ml BSA .) Storage conditions: 10 mM Tris-HCl (pH 7.5); 300 mM […]
Origin: Alteromonas haloplanktis SP Concentration: 10000 – 30000, u.a./ml Recognition site: A/CTAGT TGATC/A Reaction buffer: GM-buffer B + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; […]
Origin: E.coli strain that caries the cloned Sph I gene from Streptomyces phaeochromogenes Concentration: 5000, u.a./ml Recognition site: GCATG/C C/GTACG Reaction buffer: GM-buffer G + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT + 100 μg/ml BSA .) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 […]