Origin: Rhodopseudomonas sphaeroides Concentration: 10000 – 30000, u.a./ml Recognition site: GT/AC CA/TG Reaction buffer: GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol. Storage temperature: -20°C Ligation: After […]
Origin: E.coli strain that carries the cloned Ssp I gene from Sphaerotilus species Concentration: 10000, u.a./ml Recognition site: AAT/ATT TTA/TAA Reaction buffer: GM-buffer K + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT + 100 μg/ml BSA .) Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 […]
Origin: Rhodobacter sphaeroides 12 Concentration: 10000 – 30000, u.a./ml Recognition site: CG/GWCCG GCCWG/GC Reaction buffer: GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM […]