:Origin
Bacillus species AC

:Concentration
2000 – 5000, u.a./ml

:Recognition site
C/CGC
GGC/G

:Reaction buffer

;
GM-buffer O + BSA, (50 mM Tris-acetate (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT + 100 μg/ ml BSA.)

:Storage conditions
10 mM KH₂PO4 (pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; 200 μg/ ml BSA.

Storage temperature: -20°C

:Ligation
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
10-25	25-50	100	75-100	10-25

Product
Cat. No.	Size
E501S	100 U
E501	200 U
E502	1000 U