:Origin
E.coli strain that carries the cloned Afe I gene from Alcaligenes faecalis T2774

:Concentration
10000, u.a./ml

:Recognition site
AGC/GCT
TCG/CGA

:Reaction buffer
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

:Ligation
After 10-fold overdigestion with enzyme more than 80% of DNA pBR322 fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
10-25	25-50	75-100	75-100	100

Product
Cat. No.	Size
E213	200 U
E214	1000 U