Origin:
Vibrio nereis 18

Concentration:
10000, u.a./ml

Recognition site:
G/TGCAC
CACGT/G

Reaction buffer:
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

Ligation:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
10-25	25-50	100	25-50	25-50

Product
Cat. No.	Size
E137S	200 U
E137	1000 U
E138	5000 U