Origin:
Arthrobacter citreus
Concentration:
10000-20000, u.a./ml
Recognition site:
R/AATTY
YTTAA/R
Reaction buffer:
GM-buffer W + BSA,(10 mM Tris-HC l(pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)
Storage conditions:
20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 50% glycerol
Storage temperature: -20°C
Ligation:
After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 50 u.a. of enzyme for 16 hours at 50℃.
Optimum temperature: 50°C
Inactivation 20 minutes: under 80°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
25-50 | 50-75 | 50-75 | 100 | 10-25 |
Product | |
Cat. No. | Size |
E013S | 100 U |
E013 | 500 U |
E014 | 2500 U |