Origin:
E. coli strain that carries Vsp I gene from Vibrio species 343

Concentration:
10000, u.a./ml

Recognition site:
AT/TAAT
TAAT/TA

Reaction buffer:
GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM KPO₄ (pH 7.2); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

Ligation:
After 10-fold overdigestion with enzyme more than 70% of the DNA fragments. Of these, 90% can be ligated and recut. In the presence of 10% PEG ligation is better.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
0-10	10-25	50-75	100	25-50

Product
Cat. No.	Size
E139S	400 U
E139	1000 U
E140	5000 U