Bbs I (BstV2 I, Bbv II)

:Origin
Bacillus stearothermophilus V2

:Concentration
5000 – 15000, u.a./ml

:Recognition site
GAAGAC(N)2/
CTTCTG(N)6/

:Reaction buffer
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate ; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

:Storage temperature
-20°C

:Ligation
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55°C.

:Optimum temperature
55°C

:Inactivation 20 minutes
under 65 °C

:Enzyme activity in % of maximum

BGOWY
75-10075-10025-5025-50100
Product
Cat. No.Size
E297S100 U
E297200 U
E2981000 U