:Origin
Bacillus stearothermophilus V2
:Concentration
5000 – 15000, u.a./ml
:Recognition site
GAAGAC(N)2/
CTTCTG(N)6/
:Reaction buffer
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate ; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol
:Storage temperature
-20°C
:Ligation
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55°C.
:Optimum temperature
55°C
:Inactivation 20 minutes
under 65 °C
:Enzyme activity in % of maximum
B | G | O | W | Y |
75-100 | 75-100 | 25-50 | 25-50 | 100 |
Product: | |
Cat. No. | Size |
E297S | 100 U |
E297 | 200 U |
E298 | 1000 U |