:Origin
Bacillus stearothermophilus V1

:Concentration
1000 – 2000, u.a./ml

:Recognition site
GCAGC(N)8/
CGTCG(N)12/

:Reaction buffer
GM-buffer G , (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂ ; 50mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol

:Storage temperature
-20°C

:Ligation
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 55°C.

:Optimum temperature
55°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
75-100	100	75-100	75-100	75-100

Product：
Cat. No.	Size
E303	100 U
E304	500 U