:Origin
Bacillus globigii

:Concentration
5000 – 10000, u.a./ml

:Recognition site
GCCNNNN/NGGC
CGGN/NNNNCCG

:Reaction buffer
GM-buffer 2W , (20 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂ ; 200 mM NaCl; 1 mM DTT .)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

:Storage temperature
-20°C

:Ligation
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 65 °C

Enzyme activity in % of maximum:

B	G	O	W	Y
50-75	50-75	0-10	75-100	25-50

Product：
Cat. No.	Size
E025S	500 U
E025	1000 U
E026	5000 U