:Origin
Isolatedfrom an E.coli strain that carries the cloned Bgl I gene from Bacillus globigii

:Concentration
10000, u.a./ml

:Recognition site
A/GATCT
TCTAG/A

:Reaction buffer
GM-buffer O , (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂ ; 100 mM NaCl; 1 mM DTT .)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

:Storage temperature
-20°C

:Ligation
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 80 °C

:Enzyme activity in % of maximum

B	G	O	W	Y
0-10	10-25	100	25-50	10-25

Product：
Cat. No.	Size
E027S	500 U
E027	1000 U
E028	5000 U