Origin:
Bacillus stearothermophilus 31

Concentration:
5000, u.a./ml

Recognition site:
GGTCTC(N)₁/
CCAGAG(N)₅/

Reaction buffer:
GM-buffer O + BSA, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT + 100μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated. Of these 80% can be recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55°C.

Optimum temperature:
55°C

Inactivation 20 minutes:
under 80 °C

Enzyme activity in % of maximum:

B	G	O	W	Y
25-50	75-100	100	75-100	25-50

Product：
Cat. No.	Size
E285	100 U
E286	500 U