BsaJ I (BssEC I)

Origin:
Bacillus stearothermophilus EC

Concentration:
10000, u.a./ml

Recognition site:
C/CNNGG
GGNNC/C

Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 60°C.

Optimum temperature:
60°C

Inactivation 20 minutes:
under 80 °C

Enzyme activity in % of maximum:

BGOWY
50-7550-7550-7575-100100
Product:
Cat. No.Size
E273200 U
E2741000 U