Origin:
Pseudomonas species L

Concentration:
2000 – 5000, u.a./ml

Recognition site:
C/GTACG
GCATG/C

Reaction buffer:
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 µg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol

Storage temperature:
-20°C

Ligation:
After 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65 °C

Enzyme activity in % of maximum:

B	G	O	W	Y
75-100	75-100	25-50	10-25	100

Product：
Cat. No.	Size
E223S	100 U
E223	300 U
E224	1500 U