Bsm I (Bsa M I, Pct I)

Origin:
Planococcus citreus SM

Concentration:
10000 – 40000, u.a./ml

Recognition site:
GAATGCN/
CTTAC/GN

Reaction buffer:
GM-buffer O , (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65 °C

Enzyme activity in % of maximum:

BGOWY
25-5050-7510075-10010-25
Product
Cat. No.Size
E045S100 U
E045500 U
E0462500 U