BsmF I (BslF I)

:Origin
Bacillus stearothermophilus FI

:Concentration
1000, u.a./ml

:Recognition site
GGGAC(N)10/
CCCTG(N)14/

:Reaction buffer
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA. )

:Storage conditions
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and 95% can be recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 80 °C

:Enzyme activity in % of maximum

BGOWY
25-5025-5010-2525-50100
Product
Cat. No.Size
E479100 U
E480500 U