:Origin
Bacillus stearothermophilus FI
:Concentration
1000, u.a./ml
:Recognition site
GGGAC(N)10/
CCCTG(N)14/
:Reaction buffer
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA. )
:Storage conditions
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.
:Storage temperature
-20°C
:Ligation
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and 95% can be recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
:Optimum temperature
37°C
:Inactivation 20 minutes
under 80 °C
:Enzyme activity in % of maximum
B | G | O | W | Y |
25-50 | 25-50 | 10-25 | 25-50 | 100 |
Product: | |
Cat. No. | Size |
E479 | 100 U |
E480 | 500 U |