Bsp1286 I (Mhl I)

:Origin
Micrococcus halobius SD

:Concentration
5000 – 10000, u.a./ml

:Recognition site
GDGCH/C
C/HCGDG

:Reaction buffer
GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 80 °C

:Enzyme activity in % of maximum

BGOWY
10-2525-5075-10010010-25
Product
Cat. No.Size
E049S100 U
E049500 U
E0502500 U