:Origin
Bacillus species 13

:Concentration
10000 – 20000, u.a./ml

:Recognition site
T/CCGGA
AGGCC/T

:Reaction buffer
GM-buffer 2K, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 200 mM KCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

:Storage temperature
-20°

:Ligation
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 50°C.

:Optimum temperature
50°C

:Inactivation 20 minutes
under 65 °C

:Enzyme activity in % of maximum

B	G	O	W	Y
25-50	50-75	75-100	50-75	0-10

Product：
Cat. No.	Size
E183S	100 U
E183	500 U
E184	2500 U