:Origin
Acinetobacter calcoaceticus 36

:Concentration
2000-5000, u.a./ml

:Recognition site
ACCTGC(N)₄/
TGGACG(N)₈/

:Reaction buffer
GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 3 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
25-50	25-50	50-75	50-75	100

Product：
Cat. No.	Size
E289	100 U
E290	500 U