BsrB I (AccBS I)

:Origin
Acinetobacter calcoaceticus BS

:Concentration
5000 – 20000, u.a./ml

:Recognition site
GAG/CGG
CTC/GCC

:Reaction buffer
GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated. Of these 50% can be recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 65°C

:Enzyme activity in % of maximum

BGOWY
75-10075-10025-5025-50100
Product
Cat. No.Size
E007S200 U
E0071000 U
E0085000 U