:Origin
Acinetobacter calcoaceticus BS
:Concentration
5000 – 20000, u.a./ml
:Recognition site
GAG/CGG
CTC/GCC
:Reaction buffer
GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
:Storage temperature
-20°C
:Ligation
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated. Of these 50% can be recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
:Optimum temperature
37°C
:Inactivation 20 minutes
under 65°C
:Enzyme activity in % of maximum
B | G | O | W | Y |
75-100 | 75-100 | 25-50 | 25-50 | 100 |
Product: | |
Cat. No. | Size |
E007S | 200 U |
E007 | 1000 U |
E008 | 5000 U |