:Origin
:Bacillus stearothermophilus 3D

:Concentration
10000, u.a./ml

:Recognition site
GCAATGNN/
CGTTAC/NN

:Reaction buffer
GM-buffer G, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 50 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol

:Storage temperature
-20°C

:Ligation
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 60°C.

:Optimum temperature
60°C

:Inactivation 20 minutes
under 80 °C

:Enzyme activity in % of maximum

B	G	O	W	Y
10-25	100	25-50	50-75	75-100

Product：
Cat. No.	Size
E253	200 U
E254	1000 U