:Origin
Bacillus stearothermophilus 118

:Concentration
2000-5000, u.a./ml

:Recognition site
R/CCGGY
YGGCC/R

:Reaction buffer
GM-buffer O, (50mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM KH₂PO₄ (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

:Ligation
After 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 65°C.

:Optimum temperature

65°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
0-10	50-75	100	75-100	25-50

Product：
Cat. No.	Size
E039S	100 U
E039	200 U
E040	1000 U