:Origin
Bacillus stearothermophilus P

:Concentration
5000, u.a./ml

:Recognition site
G/CGCGC
CGCGC/G

:Reaction buffer
GM-buffer G, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 50 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 50°C.

:Optimum temperature
50°C

:Inactivation 20 minutes
under 65 °C

:Enzyme activity in % of maximum

B	G	O	W	Y
50-75	100	75-100	50-75	50-75

Product：
Cat. No.	Size
E181	200 U
E182	1000 U