:Origin
Bacillus stearothermophilus DE
:Concentration
10000 – 50000, u.a./ml
:Recognition site
C/TNAG
GANT/C
:Reaction buffer
GM-buffer G, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.
:Storage temperature
-20°C
:Ligation
After 30 -fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 60 u.a. of enzyme for 16 hours at 60°C.
:Optimum temperature
60°C
:Inactivation 20 minutes
under 80°C
:Enzyme activity in % of maximum
B | G | O | W | Y |
75-100 | 100 | 25-50 | 50-75 | 10-25 |
Product : | |
Cat. No. | Size |
E227S | 100 U |
E227 | 500 U |
E228 | 2500 U |