Hinf I

:Origin
E.coli strain that carries the cloned Hinf I gene from Haemophilus influenzae

:Concentration
20000, u.a./ml

:Recognition site
G/ANTC
CTNA/G

:Reaction buffer
GM-buffer O, (50mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 20-fold overdigestion with enzyme 90% of DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

Product
Cat. No.Size
E075S1000 U
E0755000 U
E07625000 U
BGOWY
25-5075-10010075-10075-100