Origin:
Micrococcus species 20

Concentration:
1000 – 3000, u.a./ml

Recognition site:
TGG/CCA
ACC/GGT

Reaction buffer:
GM-buffer W + BSA, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 2-fold overdigestion with enzyme 80% of DNA fragments can be ligated and recut. More complete ligation can be reached by using 10% PEG.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
50-75	50-75	25-50	100	50-75

Product：
Cat. No.	Size
E301S	20 U
E301	100 U
E302	500 U