Origin:
E.coli strain that carries the cloned Mse I gene from Thermus ruber 9

Concentration:
20000, u.a./ml

Recognition site:
T/TAA
AAT/T

Reaction buffer:
GM-buffer W, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 20-fold overdigestion with enzyme 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 65°C.

Optimum temperature:
65°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	O	W	Y
75-100	25-50	25-50	100	50-75

Product：
Cat. No.	Size
E199S	100 U
E199	500 U
E200	2500 U