Msl I (SmiM I)

Origin:
Sphingobacterium mizutae M

Concentration:
5000 – 10000, u.a./ml

Recognition site:
CAYNN/NNRTG
GTRNN/NNYAC

Reaction buffer:
GM-buffer W, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

BGOWY
10-2510-2575-10010010-25
Product
Cat. No.Size
E293S100 U
E293200 U
E2941000 U