Origin:
E.coli strain that carries the cloned Nde I gene from Flavobacterium aquatili ND
Concentration:
10000 – 20000, u.a./ml
Recognition site:
CA/TATG
GTAT/AC
Reaction buffer:
GM-buffer Y + BSA, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 10-fold overdigestion with
enzyme 80% of DNA fragments can be ligated and recut. In the presence
of 10% PEG ligation is better.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
50-75 | 75-100 | 10-25 | 50-75 | 100 |
Product: | |
Cat. No. | Size |
E009S | 400 U |
E009 | 1000 U |
E010 | 5000 U |