Origin:
Streptomyces fradiae 303

Concentration:
5000 – 20000, u.a./ml

Recognition site:
CCGC/GG
GG/CGCC

Reaction buffer:
GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
100	50-75	10-25	10-25	75-100

Product：
Cat. No.	Size
E127S	400 U
E127	1000 U
E128	5000 U