Origin:
E.coli strain that carries the cloned Sal I gene from Streptomyces albus

Concentration:
10000, u.a./ml

Recognition site:
G/TCGAC
CAGCT/G

Reaction buffer:
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl;1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	Y	W	Y
0-10	10-25	100	25-50	0-10

Product：
Cat. No.	Size
E115S	1000 U
E115	2000 U
E116	10000 U