Origin:
Planococcus citreus S
Concentration:
500 – 2000, u.a./ml
Recognition site:
GCTCTTC(N)1/
CGAGAAG(N)4/
Reaction buffer:
GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 3-fold overdigestion with enzyme
more than 90% of DNA fragments can be ligated with T4 DNA Ligase at
16°C and of these 95% can be recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 1 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
100 | 50-75 | 0-10 | 0-10 | 75-100 |
Product: | |
Cat. No. | Size |
E497 | 50 U |
E498 | 250 U |