Origin:
Isolated from Streptomyces species Bf61
Concentration:
5000, u.a./ml
Recognition site:
CCTGCA/GG
GG/ACGTCC
Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acatate; 66 mM potassium acetate; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and of these more than 90% can be recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 80°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
75-100 | 50-75 | 0-10 | 0-10 | 100 |
Product : | |
Cat. No. | Qty. |
E101 | 200 U |
E102 | 1000 U |