Origin:
Zoogloea ramigera SCA
Concentration:
10000-20000, u.a./ml
Recognition site:
AGT/ACT
TCA/TGA
Reaction buffer:
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acatate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA .)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 10-fold overdigestion with enzyme more than 70% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
| B | G | O | W | Y |
| 50-75 | 25-50 | 0-10 | 0-10 | 100 |
| Product： | |
| Cat. No. | Qty. |
| E005 | 600U |
| E006 | 3000 U |